Both teams had enjoyed remarkable comebacks during the week to reach the Copa del Rey semifinals, with Barca thrashing Sevilla 6-1 to win 8-3 on aggregate and Valencia seeing off Getafe 3-1 for a 3-2 aggregate win with two goals in stoppage time.
With 24 minutes played, a swift counter-attack ended with Rodrigo picking out Kevin Gameiro, who made no mistake from six-yards out to silence the home fans.
Barcelona nearly levelled in the 27th minute when Los Che defender Ezequiel Garay sent a Coutinho cross towards his own goal, but Neto was on hand to make an excellent save. The contact was minimal and Messi actually curled into the back of the net seconds later, but the referee had already blown for a foul.
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Valencia are one of the few teams to take points off Barca this season after they held them to a 1-1 draw in October, but they had suffered a terrible start to the season.
Messi claimed the equaliser a minute later, picking up the ball outside the area after good work from Suarez.
"We did not play well in the first half but then we showed our character and we dominated", said Barca midfielder Ivan Rakitic.
Okay Yokuslu scored 17 minutes from time to give the hosts a much-needed victory, only their sixth of the season, to end a five-match losing streak.
Rodrigo had another chance in the 63rd minute, but he turned a Cheryshev cross just wide of the post as Los Che passed up the opportunity to open up a two-goal advantage once again.
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Messi is Barcelona's talismanic striker who has caused Real players plenty of misery down the years.
In the 70th minute, Antonio Lato's knee collided with the Argentine star and he let the field for treatment.
Barcelona were unable to find a victor but they stay top of La Liga on 50 minutes.
Barcelona could be without Lionel Messi for Wednesday's Copa del Rey semi-final first leg clash against rivals Real Madrid due to a thigh injury sustained in the club's 2-2 draw with Valencia.
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